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The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5Å resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca 2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position...
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