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Direct comparison using scaffolds with a gradient pore size structure is important to investigate the effect of pore size on tissue regeneration. Collagen porous scaffolds with a pore size gradient were prepared by using pre-prepared ice particulates as a porogen material. The ice particulates had diameters of 150–250, 250–355, 355–425 and 425–500μm. The gradient collagen scaffolds had well-interconnected...
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