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SH3 is a ubiquitous domain mediating protein–protein interactions. Recent solution NMR structural studies have shown that a proline-rich peptide is capable of binding to the human vinexin SH3 domain. Here, an orthogonal amber tRNA/tRNA synthetase pair for 15 N/ 19 F-trifluoromethyl-phenylalanine ( 15 N/ 19 F-tfmF) has been applied to achieve site-specific labeling of...
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