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Comatin, an inhibitor of the non-enzymatic glycosylation (NEG) reaction, was isolated from Coprinus comatus fermentation broth by macroporous resin separation, followed by chromatographic purification using a C18 reversed-phase column. The compound was identified as 4,5-dihydroxy-2-methoxy-benzaldehyde by high-resolution MS, IR, NMR and UV analyses. The hypoglycaemic effect of comatin, on both normal...
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