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The conduction of ammonia/ammonium (NH3/NH4+) through the channel protein AmtB is inhibited by the binding of the signal transduction protein GlnK. In the AmtB–GlnK binding interface, there exists an NH3/NH4+ binding site – Am6. The calculated pK a values at the Am6 sites in both the AmtB–GlnK complex and isolated AmtB implies the dominance of an uncharged NH 3 state. The GlnK protein...
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