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Cyclohexanone monooxygenase (CHMO), a flavoenzyme of synthetic interest (it catalyses the NADPH-dependent enantioselective oxidation of ketones and of several heteroatoms such as nitrogen, sulfur, phosphorous and selenium present in organic compounds) previously overexpressed in E. coli (TOP10 pQR239), was purified to homogeneity, as demonstrated by SDS-PAGE and MALDI/TOF analysis, and characterised...
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