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Our understanding of the Bordetella pertussis secretome remains limited including the role of different growth conditions in the secretome. In this study the secretome of L1423, a clinical isolate from the 2008–2012 Australian epidemic, cultured on Stainer-Scholte (SS) and Thalen-IJssel (THIJS) media for 12h was characterised using liquid chromatography-mass spectrometry (LC-MS/MS). In the supernatant,...
Tandem mass spectrometry-based iTRAQ protein quantification provides a powerful means for identifying disease biomarkers and plays an important role in developing new diagnosis and prognosis, new treatment, and personalised medicine. However, analyses of iTRAQ data encounter a number of statistical and computational challenges such as accurate protein identification, imputation of missing values,...
The bacterium Wolinella succinogenes is the only known species of its genus. It was first isolated from cow ruminal fluid, and in cattle, it dwells in the reticulum and rumen compartments of the stomach. The global protein response of W. succinogenes to ox-bile was investigated with the aim to understand bile-tolerance mechanisms of the bacterium. Bacteria were grown in liquid media supplemented with...
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