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The catalytic site of [FeFe]-hydrogenase, the “H-cluster”, composed by a [4Fe–4S] unit connected by a cysteinyl residue to a [2Fe] center coordinated by three CO, two CN− and a bridging dithiolate, is assembled in a complex maturation pathway, at present not fully characterized, involving three conserved proteins, HydG, HydE and HydF. In this contribution we review our studies on HydF, a protein which...
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