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The disease‐associated hexameric N‐acetylglucosamine (GlcNAc)‐1‐phosphotransferase complex (α2β2γ2) catalyzes the formation of mannose 6‐phosphate residues on lysosomal enzymes required for efficient targeting to lysosomes. Using pull‐down experiments and mutant subunits, we identified a potential loop‐like region in the α‐subunits comprising residues 535–588 and 645–698 involved in the binding to...
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