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The electronic structure of Fe3+ was studied in a mutant that has been modified to bind manganese or iron at a site corresponding to the manganese-binding site of photosystem II (Kálmán et al., Biochemistry 45:13869–13874, 2006). Using electron paramagnetic resonance spectroscopy, the presence of the oxidized state of the bacteriochlorophyll dimer, P·+, was detected in the light when no metal was...
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