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The low ionic strength structures of the type III intermediate filament (IF) proteins desmin and glial fibrillary acidic protein (GFAP) have been studied by transient electric birefringence measurements. Flexible dimers with a length of around 45 nm, particles with a length of 68 ± 6 nm (presumably tetramers and hexamers) and larger aggregates of 108 ± 19 nm are found. GFAP has an increased tendency...
A selective tyrosine fluorescence quenching is found on interaction of vimentin with poly(dT) and poly(rA). However, addition of poly(dA) does not result in tyrosine quenching. The number of nucleotides covered by vimentin upon binding(n) of poly(dT) (50 +/- 4) appeared to be approximately the same as for poly(rA) (44 +/- 4), while the apparent binding constant (K app ) of the latter...
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