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Porous membrane scaffolds are widely used materials for three-dimensional cell cultures and tissue models. Additional functional modification of such scaffolds can significantly extend their use and operational performance. Here we describe hybrid microporous polystyrene-based scaffolds impregnated with a phosphorescent O 2 -sensitive dye PtTFPP, optimized for live cell fluorescence microscopy...
Multicellular spheroids are useful models of mammalian tissue for studies of cell proliferation, differentiation, replacement therapies and drug action. Having a size of 100–500 μm they mimic in vivo micro-environment and characteristic gradients of O 2 , pH and nutrients. We describe the use of cell-penetrating O 2 probes based on phosphorescent Pt-porphyrins to perform high-resolution...
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