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Uracil ist ein verbreiteter DNA‐Schaden, der aus der Hydrolyse von Cytosin resultiert und durch Uracil‐DNA‐Glycosylasen (UDGs) spezifisch beseitigt wird. Ein nichtnatürliches Pyrendesoxyribosid in kurzen DNA‐Oligomeren wurde entwickelt, das die enzymatische Aktivität von UDG direkt messen kann. Der Mechanismus beruht auf der starken Fluoreszenzlöschung des Pyrens durch Uracil, und die Aktivität des...
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