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Although fluorescein has been a traditional fluorescence dye, little reports are found about the role of H2O on its spirolactam ring dynamic balance, emission mechanism and aggregation behavior. Some reports about 5-azidofluorescein fluorescence are inaccurate. In this paper, the fluorescence of three typical compound, 5-azidofluorescein (1), 5-aminofluorescein (2) and fluorescein (3) were restudied...
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