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Macroporous chitosan layer coated on silica gel (CTS-SiO 2 ) was prepared by phase inversion and polyethylene glycol (PEG) molecular imprinting methods. Effects of chitosan and PEG content on the formation of porous surface were investigated by scanning electron microscopy (SEM). Trypsin could be covalently coupled as an affinity ligand on the macroporous chitosan layer activated with epoxy...
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