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Cross‐linking combined with MS involves enzymatic digestion of cross‐linked proteins and identifying cross‐linked peptides. Assignment of cross‐linked peptide masses requires a search of all possible binary combinations of peptides from the cross‐linked proteins’ sequences, which becomes impractical with increasing complexity of the protein system and/or if digestion enzyme specificity is relaxed...
The method of crosslinking combined with mass spectrometry is being gradually accepted as a technology enabling detailed structural information on proteins and protein complexes. Intrinsic challenges of the method, which have prevented its widespread use, are being progressively addressed by improvements in mass spectrometry instrumentation capabilities, by the development of new crosslinking reagents,...
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