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This paper describes the step-wise fabrication and characterisation of a multi-layer dual polarization interferometry (DPI) based biosensor utilising Protein G (ProG) as the bio-recognition layer for the detection of a fragment antibody (Fab′). The biosensor is capable of monitoring the concentration of Fab′ product within the extracellular medium of a fed-batch fermentation after leakage from Escherichia...
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