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ObjectivesA high histamine H1 receptor (H1R) expression cell membrane chromatography (CMC) method was developed to investigate the affinity of ligands for H1R.
MethodsThe affinity of ligands for H1R was evaluated by frontal analysis. Competition studies and molecular docking study were utilized to study the interactions that occurred at specific binding sites on H1R.
Key findingsThe KD values measured...
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