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Further study has been made of metal-catalyzed oxidation (MCO) reactions and mass spectrometry as a method to determine the binding site of copper in metalloproteins. The role of ascorbate and a variety of oxidizing agents, including O 2 , H 2 O 2 , and S 2 O 82− , have been investigated using Cu/Zn superoxide dismutase (SOD) as a model system. Ascorbate...
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