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It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re‐epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)‐based mass spectrometry was performed...
Abstract: Keratinocytes constitute the first‐line barrier against exogenous antigens and contain Toll‐like receptors (TLRs), which function as pattern‐recognition molecules to activate antimicrobial innate immune responses. In an effort to ascertain whether or not filaggrin (filament‐aggregating protein) expression affected the TLR‐mediated responses of keratinocytes, we transfected filaggrin siRNA...
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