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RNA or DNA aptamers have received much attention in recent literature as therapeutic agents and chromatographic matrices, however, their use in analytical methodologies is relatively unexplored. We describe here investigations aiming to combine this promising technology with versatile liposomes in a competitive assay format. Thus, a phospholipid derivative of an unsymmetrical 1,3-disubstituted xanthine...
The use of liposomes as analytical and bioanalytical reagents has been shown to be successful of in a variety of different applications that will be reviewed here. Due to their high surface area, large internal volume, and ability to conjugate bilayer lipids with a variety of biorecognition elements liposomes have been used in homogenous and heterogeneous assays, providing signal amplification both...
To improve the antigen-binding activity of liposome-coupled antibodies and to develop universal liposomal nanovesicles for immunoassays, protein G was conjugated to dye-loaded liposomal nanovesicles for the preparation of immunoliposomes. Sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC), a heterobifunctional cross-linker, was used to modify protein G for conjugation to...
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