To analyse the acute effects of spinal manipulation on neuromuscular function in asymptomatic individuals.Randomised controlled, cross-over trial.Spinal manipulation (SM) is used as therapeutic modality for various neuromuscular disorders and also in sport with asymptomatic individuals to improve range of motion and/or facilitate motor control. Experimental evidence of its effectiveness is lacking.27 asymptomatic participants (15 males and 12 females) [age (mean ± standard deviation) 24 ± 3 years] were exposed to three separate treatments in random order: 1) Spinal Manipulation of the lumbar spine (MAN); 2) Stretching of the Lumbar spine (STR); 3) sham manipulation (SHA).Before (PRE), after (POST) and 15 min after (15_MIN) each treatment, the participants were asked to perform three tasks always in the same order: 1) force fluctuation task; 2) Modified Sörensen’s test; 3) sit and reach. Surface EMG was recorded from Gastrocnemius medialis and Erector Spinae muscles using linear arrays during task 1 and 2.MAN was not shown to determine improvements superior to other treatments in the control of force output and sEMG parameters.Studies with larger populations are needed in order to ascertain the effectiveness of SM on neuromuscular function.