Objective Evaluations of trials of the effectiveness of dietary intervention programs may be compromised by response set biases, such as those attributable to social desirability. Participants who receive a behavioral intervention may bias their reports of diet to appear in compliance with intervention goals. This study examined whether responses to standard dietary assessment instruments could be affected by a brief dietary intervention. Design We assigned 192 undergraduate students randomly to (a) see a 17-minute videotape on the consequences of eating a high-fat diet or a placebo videotape on workplace management and (b) receive preintervention and post-intervention assessments or only postintervention assessment. Dietary assessments included 4 independent measures of fat intake. Results Among women, bias (intervention minus control) was −9.7 g fat (from a short food frequency questionnaire) and −0.6 high-fat foods (from a questionnaire about use of 23 foods in the previous day) (P<.05 for both). No results were significant among men or for 2 instruments that measured more qualitative aspects of fat-related dietary habits. Applications Even a modest dietary intervention can affect responses to dietary assessment instruments. Nutritionists should recognize that assessment of adherence to dietary change recommendations, when based on dietary self-report, can be overestimated as a result of response set biases. J Am Diet Assoc. 1998;98:40-43.