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Chemical allergens bind directly, or after metabolic or abiotic activation, to endogenous proteins to become allergenic. Assessment of this initial binding has been suggested as a target for development of assays to screen chemicals for their allergenic potential. Recently we reported a nitrobenzenethiol (NBT) based method for screening thiol reactive skin sensitizers, however, amine selective sensitizers...
We propose to use infrared light coupled with a near field scanning optical microscope (NSOM) to probe organic materials. The initial emphasis will be on the 2.8 – 3.25 µm range, which contains bands from both C - H and O - H stretching vibrations. This provides great sensitivity to specific chemical alterations as induced, e.g., in a photoresist by exposure and/or development. We have attempted to...
Controlled deposition of metal nanoparticles (NPs) into lithographic patterned nanoholes has been a major challenge. Electrostatic attraction was employed in directing gold NPs of precise size into e-beam lithographically patterned holes. Citrate stabilized gold NPs with a net negative surface charge were deposited on a positively charged well-bottom surface generated by adsorption of long-chain amino-terminated...
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