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4‐Substitution on proline directly impacts protein main chain conformational preferences. The structural effects of N‐acyl substitution and of 4‐substitution were examined by NMR spectroscopy and X‐ray crystallography on minimal molecules with a proline 4S‐nitrobenzoate. The effects of N‐acyl substitution on conformation were attenuated in the 4S‐nitrobenzoate context, due to the minimal role of the...
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