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Reconstruction and optimization of biosynthetic pathways can help to overproduce target chemicals in microbial cell factories based on genetic engineering. However, the perturbation of biosynthetic pathways on cellular metabolism is not well investigated and profiling the engineered microbes remains challenging. The rapid development of omics tools has the potential to characterize the engineered...
α-Amylases (1,4-α-D-glucanohydrolases) are widely used in starch liquefaction, but their acid stability needs to be continuously explored to reduce the costs of raw material and operation. In this study, to better meet the industrial requirements, the acid stability of Bacillus licheniformis α-amylase (BLA) was further improved by directed evolution using error prone polymerase chain reaction (PCR)...
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