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article

Visceral pain – Novel approaches for optogenetic control of spinal afferents

Nick J. Spencer, Tim J. Hibberd, Malin Lagerström, Yoichiro Otsuka, more

Brain Research > 2018 > 1693 > PB > 159-164

Painful stimuli arising within visceral organs are detected by peripheral nerve endings of spinal afferents, whose cell bodies are located in dorsal root ganglia (DRG). Recent technical advances have made it possible to reliably expose and inject single DRG with neuronal tracers or viruses in vivo. This has facilitated, for the first time, unequivocal identification of different types of spinal afferent...

article

Light‐induced fermenter production of derivatives of the sweet protein monellin is maximized in prestationary Saccharomyces cerevisiae cultures

Simona Gramazio, Jonathan Trauth, Filipp Bezold, Lars‐Oliver Essen, more

Biotechnology Journal > 17 > 8 > n/a - n/a

Optogenetics has great potential for biotechnology and metabolic engineering due to the cost‐effective control of cellular activities. The usage of optogenetics techniques for the biosynthesis of bioactive molecules ensures reduced costs and enhanced regulatory possibilities. This requires development of efficient methods for light‐delivery during a production process in a fermenter. Here, we benchmarked...

article

Peripheral chemoreflex activation induces expiratory but not inspiratory excitation of C1 pre‐sympathetic neurones of rats

Melina P. Silva, Pedro F. Spiller, Julian F. R. Paton, Davi J. A. Moraes

Acta Physiologica > 235 > 4 > n/a - n/a

Aims Stimulation of peripheral chemoreceptors, as during hypoxia, increases breathing and respiratory‐related sympathetic bursting. Activation of catecholaminergic C1 neurones induces sympathoexcitation, while its ablation reduces the chemoreflex sympathoexcitatory response. However, no study has determined the respiratory phase(s) in which the pre‐sympathetic C1 neurones are recruited by peripheral...

article

Connectivity and synaptic features of hilar mossy cells and their effects on granule cell activity along the hippocampal longitudinal axis

Wahab Imam Abdulmajeed, Kai‐Yi Wang, Jei‐Wei Wu, Musa Iyiola Ajibola, more

The Journal of Physiology > 600 > 14 > 3355 - 3381

Abstract The hippocampus is an elongated brain structure which runs along a ventral‐to‐dorsal axis in rodents, corresponding to the anterior‐to‐posterior axis in humans. A glutamatergic cell type in the dentate gyrus (DG), the mossy cells (MCs), establishes extensive excitatory collateral connections with the DG principal cells, the granule cells (GCs), and inhibitory interneurons in both hippocampal...

article

Novel vision restoration techniques: 3D bioprinting, gene and stem cell therapy, optogenetics, and the bionic eye

Abhay Menon, Sanjairaj Vijayavenkataraman

Artificial Organs > 46 > 8 > 1463 - 1474

Background Vision restoration has been one of the most sought‐after goals of ophthalmology because of its inception. Despite these problems being tackled from numerous different perspectives, a concrete solution has not yet been achieved. An optimal solution will have significant implications on the patient's quality of life, socioeconomic status, and mental health. Methods This article will explore...

article

Holographic optogenetic stimulation with calcium imaging as an all optical tool for cardiac electrophysiology

Sebastian Junge, Felix Schmieder, Philipp Sasse, Jürgen Czarske, more

Journal of Biophotonics > 15 > 7 > n/a - n/a

All optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple...

article

Insights on gastrointestinal motility through the use of optogenetic sensors and actuators

Bernard T. Drumm, Caroline A. Cobine, Salah A. Baker

The Journal of Physiology > 600 > 13 > 3031 - 3052

The muscularis of the gastrointestinal (GI) tract consists of smooth muscle cells (SMCs) and various populations of interstitial cells of Cajal (ICC), platelet‐derived growth factor receptor α⁺ (PDGFRα⁺) cells, as well as excitatory and inhibitory enteric motor nerves. SMCs, ICC and PDGFRα⁺ cells form an electrically coupled syncytium, which together with inputs from the enteric nervous system (ENS)...

article

Pathway‐Specific Remodeling of Thalamostriatal Synapses in a Mouse Model of Parkinson's Disease

Asami Tanimura, Weixing Shen, David Wokosin, D. James Surmeier

Movement Disorders > 37 > 6 > 1164 - 1174

Background The network pathophysiology underlying the motor symptoms of Parkinson's disease (PD) is poorly understood. In models of late‐stage PD, there is significant cell‐specific remodeling of corticostriatal, axospinous glutamatergic synapses on principal spiny projection neurons (SPNs). Neurons in the centrolateral nucleus (CLN) of the thalamus that relay cerebellar activity to the striatum...

article

A fast motivated tail in the ventral tegmental area

Melissa J. Chee

The Journal of Physiology > 600 > 11 > 2547 - 2548

article

Optogenetic tools for manipulation of cyclic nucleotides functionally coupled to cyclic nucleotide‐gated channels

Thilo Henß, Jatin Nagpal, Shiqiang Gao, Ulrike Scheib, more

British Journal of Pharmacology > 179 > 11 > 2519 - 2537

Background and Purpose The cyclic nucleotides cAMP and cGMP are ubiquitous second messengers regulating numerous biological processes. Malfunctional cNMP signalling is linked to diseases and thus is an important target in pharmaceutical research. The existing optogenetic toolbox in Caenorhabditis elegans is restricted to soluble adenylyl cyclases, the membrane‐bound Blastocladiella emersonii CyclOp...

article

Biobased Elastomer Nanofibers Guide Light‐Controlled Human‐iPSC‐Derived Skeletal Myofibers

Aimee Cheesbrough, Fabiola Sciscione, Federica Riccio, Peter Harley, more

Advanced Materials > 34 > 18 > n/a - n/a

Generating skeletal muscle tissue that mimics the cellular alignment, maturation, and function of native skeletal muscle is an ongoing challenge in disease modeling and regenerative therapies. Skeletal muscle cultures require extracellular guidance and mechanical support to stabilize contractile myofibers. Existing microfabrication‐based solutions are limited by complex fabrication steps, low throughput,...

article

Processing of information from the parafascicular nucleus of the thalamus through the basal ganglia

Maroua Hanini‐Daoud, Florence Jaouen, Pascal Salin, Lydia Kerkerian‐Le Goff, more

Journal of Neuroscience Research > 100 > 6 > 1370 - 1385

Accumulating evidence implicates the parafascicular nucleus of the thalamus (Pf) in basal ganglia (BG)‐related functions and pathologies. Despite Pf connectivity with all BG components, most attention is focused on the thalamostriatal system and an integrated view of thalamic information processing in this network is still lacking. Here, we addressed this question by recording the responses elicited...

article

Distinct limbic dopamine regulation across olfactory‐tubercle subregions through integration of in vivo fast‐scan cyclic voltammetry and optogenetics

Rohan V. Bhimani, Ryan Yates, Caroline E. Bass, Jinwoo Park

Journal of Neurochemistry > 161 > 1 > 53 - 68

The olfactory tubercle (OT), an important component of the ventral striatum and limbic system, is involved in multi‐sensory integration of reward‐related information in the brain. However, its functional roles are often overshadowed by the neighboring nucleus accumbens. Increasing evidence has highlighted that dense dopamine (DA) innervation of the OT from the ventral tegmental area (VTA) is implicated...

article

Tailoring Organic LEDs for Bidirectional Optogenetic Control via Dual‐Color Switching

Giuseppe Ciccone, Ilenia Meloni, Rodrigo G. Fernandez Lahore, Johannes Vierock, more

Advanced Functional Materials > 32 > 12 > n/a - n/a

Revealing the intricate logic of neuronal circuits and its connection to the physiopathology of living systems constitutes a fundamental question in neuroscience. Optogenetics offers the possibility to use light of specific wavelengths to study the activity of neurons with unprecedented spatiotemporal resolution. To make use of this technique at its full potential, bidirectional proteins may be expressed...

article

Optogenetic stimulation of ventral tegmental area dopaminergic neurons in a female rodent model of depression: The effect of different stimulation patterns

Yixin Tong, Lisa Pfeiffer, Tsvetan Serchov, Volker A. Coenen, more

Journal of Neuroscience Research > 100 > 3 > 897 - 911

Major depressive disorder is one of the most common mental disorders, and more than 300 million of people suffer from depression worldwide. Recent clinical trials indicate that deep brain stimulation of the superolateral medial forebrain bundle (mfb) can have rapid and long‐term antidepressant effects in patients with treatment‐resistant depression. However, the mechanisms of action are elusive. In...

article

Rapid directed molecular evolution of fluorescent proteins in mammalian cells

Siranush Babakhanova, Erica E. Jung, Kazuhiko Namikawa, Hanbin Zhang, more

Protein Science > 31 > 3 > 728 - 751

In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian cells. Using this method, we were able to perform screening of large gene libraries containing up to 2 × 10⁷ independent random genes of fluorescent...

article

The state of the art of biomedical applications of optogenetics

Hoda Keshmiri Neghab, Mohammad Hasan Soheilifar, Michael Grusch, Manoela Marques Ortega, more

Lasers in Surgery and Medicine > 54 > 2 > 202 - 216

Background and objective Optogenetics has opened new insights into biomedical research with the ability to manipulate and control cellular activity using light in combination with genetically engineered photosensitive proteins. By stimulating with light, this method provides high spatiotemporal and high specificity resolution, which is in contrast to conventional pharmacological or electrical stimulation...

article

Phase‐ and state‐dependent modulation of breathing pattern by preBötzinger complex somatostatin expressing neurons

Raquel P. Sousa Abreu, Evgeny Bondarenko, Jack L. Feldman

The Journal of Physiology > 600 > 1 > 143 - 165

Abstract As neuronal subtypes are increasingly categorized, delineating their functional role is paramount. The preBötzinger complex (preBötC) subpopulation expressing the neuropeptide somatostatin (SST) is classified as mostly excitatory, inspiratory‐modulated and not rhythmogenic. We further characterized their phenotypic identity: 87% were glutamatergic and the balance were glycinergic and/or...

article

Adolescent social isolation induced alterations in nucleus accumbens glutamate signalling

Andre U. Deutschmann, Julia M. Kirkland, Lisa A. Briand

Addiction Biology > 27 > 1 > n/a - n/a

Exposure to adversity during early childhood and adolescence increases an individual's vulnerability to developing substance use disorder. Despite the knowledge of this vulnerability, the mechanisms underlying it are still poorly understood. Excitatory afferents to the nucleus accumbens (NAc) mediate responses to both stressful and rewarding stimuli. Understanding how adolescent social isolation alters...

article

Repetitive short‐pulsed illumination efficiently activates photoactivatable‐Cre as continuous illumination in embryonic stem cells and pre‐implantation embryos of transgenic mouse

Kanae Kishi, Hiroshi Koyama, Sanae Oka, Azusa Kato, more

genesis > 59 > 12 > n/a - n/a

The Cre‐loxP system has been widely used for specific DNA recombination which induces gene inactivation or expression. Recently, photoactivatable‐Cre (PA‐Cre) proteins have been developed as a tool for spatiotemporal control of the enzymatic activity of Cre recombinase. Here, we generated transgenic mice bearing a PA‐Cre gene and systematically investigated the conditions of photoactivation for the...

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Search results

Visceral pain – Novel approaches for optogenetic control of spinal afferents

Light‐induced fermenter production of derivatives of the sweet protein monellin is maximized in prestationary Saccharomyces cerevisiae cultures

Peripheral chemoreflex activation induces expiratory but not inspiratory excitation of C1 pre‐sympathetic neurones of rats

Connectivity and synaptic features of hilar mossy cells and their effects on granule cell activity along the hippocampal longitudinal axis

Novel vision restoration techniques: 3D bioprinting, gene and stem cell therapy, optogenetics, and the bionic eye

Holographic optogenetic stimulation with calcium imaging as an all optical tool for cardiac electrophysiology

Insights on gastrointestinal motility through the use of optogenetic sensors and actuators

Pathway‐Specific Remodeling of Thalamostriatal Synapses in a Mouse Model of Parkinson's Disease

A fast motivated tail in the ventral tegmental area

Optogenetic tools for manipulation of cyclic nucleotides functionally coupled to cyclic nucleotide‐gated channels

Biobased Elastomer Nanofibers Guide Light‐Controlled Human‐iPSC‐Derived Skeletal Myofibers

Processing of information from the parafascicular nucleus of the thalamus through the basal ganglia

Distinct limbic dopamine regulation across olfactory‐tubercle subregions through integration of in vivo fast‐scan cyclic voltammetry and optogenetics

Tailoring Organic LEDs for Bidirectional Optogenetic Control via Dual‐Color Switching

Optogenetic stimulation of ventral tegmental area dopaminergic neurons in a female rodent model of depression: The effect of different stimulation patterns

Rapid directed molecular evolution of fluorescent proteins in mammalian cells

The state of the art of biomedical applications of optogenetics

Phase‐ and state‐dependent modulation of breathing pattern by preBötzinger complex somatostatin expressing neurons

Adolescent social isolation induced alterations in nucleus accumbens glutamate signalling

Repetitive short‐pulsed illumination efficiently activates photoactivatable‐Cre as continuous illumination in embryonic stem cells and pre‐implantation embryos of transgenic mouse

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