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Items from 1 to 20 out of 15,185 results

article

Effect of Piper betel leaf stalk extract on protein metabolism in reproductive tissues of male albino rats

V Vengaiah, A Govardhan Naik, C Changamma, V. Vengaiah, more

Asian Pacific Journal of Reproduction > 2015 > 4 > 2 > 91-95

Objective: To know the impact of Piper betel leaf stalk (P. betel) extract on Protein and energy metabolism and its role in male albino rats. Methods: Healthy adult (3-4 months old) male Wistar strain albino rats were administered with betel leaf stalk extract, at the dose of 50 mg/kg/day through oral gavages for 15 days. Twenty four hours after the last dose, the animals were autopsied. In order...

article

A comparative study of physical pretreatments for the extraction of polyphenols and proteins from vine shoots

Hiba N. Rajha, Nadia Boussetta, Nicolas Louka, Richard G. Maroun, more

Food Research International > 2014 > 65 > Part C > 462-468

This work examined the potential of valorization of vine shoots through their polyphenol and protein contents. However the choice of the experimental conditions targeted polyphenol extraction at the expense of proteins for further simplification of the purification process. The intensification of polyphenol and protein extraction by physical treatments (pulsed electric fields (PEF), high-voltage electrical...

article

Anti-inflammatory and antioxidant activities, functional properties and mutagenicity studies of protein and protein hydrolysate obtained from Prosopis alba seed flour

Florencia Cattaneo, Jorge Esteban Sayago, María Rosa Alberto, Iris Catiana Zampini, more

Food Chemistry > 2014 > 161 > Complete > 391-399

Prosopis species are considered multipurpose trees and shrubs by FAO and their fruit constitute a food source for humans and animals. According to the ''Codigo Alimentario Argentino'', ''algarrobo flour'' is produced by grinding the whole mature pod, but in the traditional process most of the seeds are discarded. In this paper, the flour from seed was obtained. Then, the proteins were extracted and...

article

Model cell membranes: Discerning lipid and protein contributions in shaping the cell

Thomas Günther Pomorski, Tommy Nylander, Marité Cárdenas, T.G. Pomorski, more

Advances in Colloid and Interface Science > 2014 > 205 > Complete > 207-220

The high complexity of biological membranes has motivated the development and application of a wide range of model membrane systems to study biochemical and biophysical aspects of membranes in situ under well defined conditions. The aim is to provide fundamental understanding of processes controlled by membrane structure, permeability and curvature as well as membrane proteins by using a wide range...

article

Biopharmaceutical applications of calixarenes

E. Da Silva, A.N. Lazar, A.W. Coleman, E. Da Silva, more

Journal of Drug Delivery Science and Technology > 2004 > 14 > 1 > 3-20

During the last 15 years, water soluble calixarenes and especially para-sulphonato-calix[n]arene and amphiphilic calixarenes have been the subject of growing interest in the biological domain. This article provides an overview of the studies, by our group and other research groups, that led to the elucidation of biological and biopharmaceutical activity of these calixarenes. The different studies...

article

Specific Signal Enhancement on an RNA‐Protein Interface by Dynamic Nuclear Polarization

Victoria Aladin, Arun K. Sreemantula, Thomas Biedenbänder, Alexander Marchanka, more

Chemistry – A European Journal > 29 > 16 > n/a - n/a

Sensitivity and specificity are both crucial for the efficient solid‐state NMR structure determination of large biomolecules. We present an approach that features both advantages by site‐specific enhancement of NMR spectroscopic signals from the protein‐RNA binding site within a ribonucleoprotein (RNP) by dynamic nuclear polarization (DNP). This approach uses modern biochemical techniques for sparse...

article

Front Cover: Specific Signal Enhancement on an RNA‐Protein Interface by Dynamic Nuclear Polarization (Chem. Eur. J. 16/2023)

Victoria Aladin, Arun K. Sreemantula, Thomas Biedenbänder, Alexander Marchanka, more

Chemistry – A European Journal > 29 > 16 > n/a - n/a

Like bright stars in a dark sky, sparsely ¹³C‐labeled methyl groups in the protein domain “illuminate” the binding pocket of a ribonucleoprotein (RNP) complex for specific solid‐state NMR spectroscopy of the interaction surface by localized transfer of their hyperpolarization to ¹³C‐labeled adenosines in the RNA domain. This is enabled by specific cross‐relaxation enhancement by active motions under...

article

Investigation on the emulsification mechanism in aqueous enzymatic extraction of edible oil from Schizochytrium sp.

Xu Zheng, Ma Juan, Xiaohong Kou, Xin Gao, more

Journal of the Science of Food and Agriculture > 103 > 6 > 2904 - 2913

BACKGROUND The interaction between emulsified substances and lipids generates an emulsification system during the extraction of microalgae edible oil by aqueous enzymatic method. This study aimed to resolve the dynamics of interfacial protein adsorption during the extraction of microalgae oil at different enzymatic times and the effect on the stability of the interfacial membrane formed by the proteins...

article

Exploring proteomic plasma biomarkers in eosinophilic and neutrophilic asthma

Maura Kere, Susanna Klevebro, Natalia Hernandez‐Pacheco, Maria Ödling, more

Clinical & Experimental Allergy > 53 > 2 > 186 - 197

Background Few biomarkers identify eosinophilic and neutrophilic asthma beyond cell concentrations in blood or sputum. Finding novel biomarkers for asthma endotypes could give insight about disease mechanisms and guide tailored treatment. Our aim was to investigate clinical characteristics and inflammation‐related plasma proteins in relation to blood eosinophil and neutrophil concentrations in subjects...

article

Molecular Rotational Correlation Times and Nanoviscosity Determined by ^111mCd Perturbed Angular Correlation (PAC) of γ‐rays Spectroscopy

Rasmus Fromsejer, Marianne L. Jensen, Matthew O. Zacate, Victoria L. Karner, more

Chemistry – A European Journal > 29 > 9 > n/a - n/a

The nanoviscosity experienced by molecules in solution may be determined through measurement of the molecular rotational correlation time, τ_c, for example, by fluorescence and NMR spectroscopy. With this work, we apply PAC spectroscopy to determine the rate of rotational diffusion, λ=1/τ_c, of a de novo designed protein, TRIL12AL16C, in solutions with viscosities, ξ, from 1.7 to 88 mPa⋅s. TRIL12AL16C...

article

Design of Stimuli‐Responsive Peptides and Proteins

Yang Li, Guangze Yang, Lukas Gerstweiler, San H. Thang, more

Advanced Functional Materials > 33 > 7 > n/a - n/a

Stimuli‐responsive peptides and proteins are an exciting class of smart biomaterials for various applications and have received significant attention over the past decades. A wide variety of stimuli such as temperature, pH, ions, enzymes, magnetic field, redox, etc., are explored. This article provides a review of five intensively studied types of stimuli‐responsive peptides and proteins, their design...

article

Chloroacetamide‐Modified Nucleotide and RNA for Bioconjugations and Cross‐Linking with RNA‐Binding Proteins

Mária Brunderová, Matouš Krömer, Marta Vlková, Michal Hocek

Angewandte Chemie International Edition > 62 > 7 > n/a - n/a

Reactive RNA probes are useful for studying and identifying RNA‐binding proteins. To that end, we designed and synthesized chloroacetamide‐linked 7‐deaza‐ATP which was a good substrate for T7 RNA polymerase in in vitro transcription assay to synthesize reactive RNA probes bearing one or several reactive modifications. Modified RNA probes reacted with thiol‐containing molecules as well as with cysteine‐...

article

An Engineered OmpG Nanopore with Displayed Peptide Motifs for Single‐Molecule Multiplex Protein Detection

Joshua C. Foster, Bach Pham, Ryan Pham, Minji Kim, more

Angewandte Chemie International Edition > 62 > 7 > n/a - n/a

Molecular detection via nanopore, achieved by monitoring changes in ionic current arising from analyte interaction with the sensor pore, is a promising technology for multiplex sensing development. Outer Membrane Protein G (OmpG), a monomeric porin possessing seven functionalizable loops, has been reported as an effective sensing platform for selective protein detection. Using flow cytometry to screen...

article

An Engineered OmpG Nanopore with Displayed Peptide Motifs for Single‐Molecule Multiplex Protein Detection

Joshua C. Foster, Bach Pham, Ryan Pham, Minji Kim, more

Angewandte Chemie > 135 > 7 > n/a - n/a

article

Chloroacetamide‐Modified Nucleotide and RNA for Bioconjugations and Cross‐Linking with RNA‐Binding Proteins

Mária Brunderová, Matouš Krömer, Marta Vlková, Michal Hocek

Angewandte Chemie > 135 > 7 > n/a - n/a

article

Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging

Fabio Moliner, Zuzanna Konieczna, Lorena Mendive‐Tapia, Rebecca S. Saleeb, more

Angewandte Chemie International Edition > 62 > 4 > n/a - n/a

The multiple applications of super‐resolution microscopy have prompted the need for minimally invasive labeling strategies for peptide‐guided fluorescence imaging. Many fluorescent reporters display limitations (e.g., large and charged scaffolds, non‐specific binding) as building blocks for the construction of fluorogenic peptides. Herein we have built a library of benzodiazole amino acids and systematically...

article

Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging

Fabio Moliner, Zuzanna Konieczna, Lorena Mendive‐Tapia, Rebecca S. Saleeb, more

Angewandte Chemie > 135 > 4 > n/a - n/a

article

Bioinspired Fluorescent Polymers: Synthesis, Processing, and Applications^†

Hongxue Xu, Wei Zhang, Mingfeng Wang

Chinese Journal of Chemistry > 41 > 4 > 458 - 468

The discovery of fluorescent proteins has inspired a lot of interest to develop synthetic polymers with similar structures and functionalities. In this review article, we first give a brief introduction for the discovery, the structural characteristics and optical properties of fluorescent proteins, using green fluorescent protein (GFP) as an example. In the body part of article, we summarize recent...

article

Understanding the helical stability of charged peptides

Nitin Kumar Singh, Manish Agarwal, Mithun Radhakrishna

Proteins: Structure, Function, and Bioinformatics > 91 > 2 > 268 - 276

Cationic helical peptides play a crucial role in applications such as anti‐microbial and anticancer activity. The activity of these peptides directly correlates with their helicity. In this study, we have performed extensive all‐atom molecular dynamics simulations of 25 Lysine–Leucine co‐polypeptide sequences of varying charge density (

λ

) and patterns. Our findings showed that, an increase in the...

article

Foliar application of wood distillate boosts plant yield and nutritional parameters of chickpea

Riccardo Fedeli, Andrea Vannini, Silvia Celletti, Viviana Maresca, more

Annals of Applied Biology > 182 > 1 > 57 - 64

In the quest for eco‐friendly products with biostimulant properties, foliar application of wood distillate (WD) was tested on the growth and yield of chickpea (Cicer arietinum L.). WD (pyroligneous acid) is a by‐product of plant biomass pyrolysis and is rich in biologically active substances like polyphenols, alcohols, acids and esters. In this work, chickpea plants were sprayed weekly with 100 ml...

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Search results

Effect of Piper betel leaf stalk extract on protein metabolism in reproductive tissues of male albino rats

A comparative study of physical pretreatments for the extraction of polyphenols and proteins from vine shoots

Anti-inflammatory and antioxidant activities, functional properties and mutagenicity studies of protein and protein hydrolysate obtained from Prosopis alba seed flour

Model cell membranes: Discerning lipid and protein contributions in shaping the cell

Biopharmaceutical applications of calixarenes

Specific Signal Enhancement on an RNA‐Protein Interface by Dynamic Nuclear Polarization

Front Cover: Specific Signal Enhancement on an RNA‐Protein Interface by Dynamic Nuclear Polarization (Chem. Eur. J. 16/2023)

Investigation on the emulsification mechanism in aqueous enzymatic extraction of edible oil from Schizochytrium sp.

Exploring proteomic plasma biomarkers in eosinophilic and neutrophilic asthma

Molecular Rotational Correlation Times and Nanoviscosity Determined by ^111mCd Perturbed Angular Correlation (PAC) of γ‐rays Spectroscopy

Design of Stimuli‐Responsive Peptides and Proteins

Chloroacetamide‐Modified Nucleotide and RNA for Bioconjugations and Cross‐Linking with RNA‐Binding Proteins

An Engineered OmpG Nanopore with Displayed Peptide Motifs for Single‐Molecule Multiplex Protein Detection

An Engineered OmpG Nanopore with Displayed Peptide Motifs for Single‐Molecule Multiplex Protein Detection

Chloroacetamide‐Modified Nucleotide and RNA for Bioconjugations and Cross‐Linking with RNA‐Binding Proteins

Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging

Small Fluorogenic Amino Acids for Peptide‐Guided Background‐Free Imaging

Bioinspired Fluorescent Polymers: Synthesis, Processing, and Applications^†

Understanding the helical stability of charged peptides

Foliar application of wood distillate boosts plant yield and nutritional parameters of chickpea

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