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Cryopreservation of chicken blastodermal cells and their quality assessment by flow cytometry and transmission electron microscopy

Andrea Svoradová, Lenka Kuželová, Jaromír Vašíček, Lucia Olexíková, more

Biotechnology Progress > 34 > 3 > 778 - 783

The goal of this study was to evaluate effect of slow freezing and vitrification methods on the viability of chicken blastodermal cells (BCs). Proper aliquot of isolated BCs were diluted in the freezing medium composed of 10% DMSO and frozen in the freezing vessel BICELL to reach desired temperature up to −80°C. Then samples were immersed in liquid nitrogen. Other cell aliquot was vitrified in solution...

article

Polyclonal, newly derived T cells with low expression of inhibitory molecule PD-1 in tonsils define the phenotype of lymphocytes in children with Periodic Fever, Aphtous Stomatitis, Pharyngitis and Adenitis (PFAPA) syndrome

Dytrych Petra, Krol Petra, Kotrova Michaela, Kuzilkova Daniela, more

Molecular Immunology > 2015 > 65 > 1 > 139-147

Purpose: PFAPA syndrome is a benign, recurrent inflammatory disease of childhood. Tonsillectomy is one of the therapeutic options with a yet unexplained biological mechanism. We tested whether specific lymphocyte subsets recruited from blood to human tonsils participate in PFAPA pathogenesis. Methods: Paired tonsils/peripheral blood (PB) samples were investigated (a) from children with PFAPA that...

article

The characterisation and summer distribution of ultraphytoplankton in the Gulf of Gabès (Eastern Mediterranean Sea, Tunisia) by using flow cytometry

I. Hamdi, M. Denis, A. Bellaaj-Zouari, H. Khemakhem, more

Continental Shelf Research > 2015 > 93 > Complete > 27-38

The Gulf of Gabès in the Eastern Mediterranean Basin is one of the largest continental shelves and is a highly productive area. This is in contrast to the Eastern Mediterranean Basin as a whole which is oligotrophic to ultraoligotrophic. Over recent years, due to increased pressure from anthropological activities, there has been a decline in the biological resources in the Gulf of Gabès. This has...

article

Joint analysis of flow cytometry data and fluorescence spectra as a non-negative array factorization problem

David Brie, Rémi Klotz, Sebastian Miron, Saïd Moussaoui, more

Chemometrics and Intelligent Laboratory Systems > 2014 > 137 > Complete > 21-32

The paper presents a novel approach to the processing of flow cytometry data sequences. It consists in decomposing a sequence of multidimensional probability density functions by using the multilinear block tensor decomposition approach [1,2]. Also a formal link between flow cytometry data and fluorescence spectra is provided allowing the joint processing of both data. To illustrate the effectiveness...

article

An efficient and rapid method for the enumeration of heterotrophic prokaryotes in coastal sediments by flow cytometry

Céline Lavergne, Laureen Beaugeard, Christine Dupuy, Claude Courties, more

Journal of Microbiological Methods > 2014 > 105 > Complete > 31-38

Flow cytometry offers an easy and powerful way to assess multi-parametric data in different domains, notably in the environmental sciences. Because evaluating heterotrophic prokaryotic abundance is crucial to understand an ecosystem's functioning, we propose a quick and efficient protocol for (1) cell's detachment in muddy coastal sediments followed by (2) enumeration of prokaryotes by flow cytometry...

article

Assessment of Daucus carota L. (Apiaceae) subspecies by chemotaxonomic and DNA content analyses

Ana Cristina Tavares, João Loureiro, Sílvia Castro, António P. Coutinho, more

Biochemical Systematics and Ecology > 2014 > 55 > Complete > 222-230

For a clearer distinction between the four subspecies of Daucus carota native from Portugal (subsp. carota, subsp. maximus, subsp. gummifer and subsp. halophilus), morphological features of the fruits, DNA content analyses by flow cytometry, and chemical characterization of the essential oils were undertaken.We found chemotaxonomic evidences to consider D. carota subsp. maximus as a separate species...

article

Flow cytometric assessment of microbial abundance in the near-field area of seawater reverse osmosis concentrate discharge

R. van der Merwe, F. Hammes, S. Lattemann, G. Amy, more

Desalination > 2014 > 343 > Complete > 208-216

The discharge of concentrate and other process waters from seawater reverse osmosis (SWRO) plant operations into the marine environment may adversely affect water quality in the near-field area surrounding the outfall. The main concerns are the increase in salt concentration in receiving waters, which results in a density increase and potential water stratification near the outfall, and possible increases...

article

Impact of cold plasma on Citrobacter freundii in apple juice: Inactivation kinetics and mechanisms

Björn Surowsky, Antje Fröhling, Nathalie Gottschalk, Oliver Schlüter, more

International Journal of Food Microbiology > 2014 > 174 > Complete > 63-71

Various studies have shown that cold plasma is capable of inactivating microorganisms located on a variety of food surfaces, food packaging materials and process equipment under atmospheric pressure conditions; however, less attention has been paid to the impact of cold plasma on microorganisms in liquid foodstuffs.The present study investigates cold plasma's ability to inactivate Citrobacter freundii...

article

Optimization of murine small intestine leukocyte isolation for global immune phenotype analysis

Andrew W. Goodyear, Ajay Kumar, Steven Dow, Elizabeth P. Ryan, more

Journal of Immunological Methods > 2014 > 405 > Complete > 97-108

New efforts to understand complex interactions between diet, gut microbiota, and intestinal immunity emphasize the need for a standardized murine protocol that has been optimized for the isolation of lamina propria immune cells. In this study multiple mouse strains including BALB/c, 129S6/Sv/EvTac and ICR mice were utilized to develop an optimal protocol for global analysis of lamina propria leukocytes...

article

Recovery of soil unicellular eukaryotes: An efficiency and activity analysis on the single cell level

Guillaume Lentendu, Thomas Hübschmann, Susann Müller, Susanne Dunker, more

Journal of Microbiological Methods > 2013 > 95 > 3 > 463-469

Eukaryotic unicellular organisms are an important part of the soil microbial community, but they are often neglected in soil functional microbial diversity analysis, principally due to the absence of specific investigation methods in the special soil environment. In this study we used a method based on high-density centrifugation to specifically isolate intact algal and yeast cells, with the aim to...

article

The spatio-ecological segregation of different cytotypes of Oxalis obtusa (Oxalidaceae) in contact zones

J. Krejčíková, R. Sudová, K. Oberlander, L.L. Dreyer, more

South African Journal of Botany > 2013 > 88 > Complete > 62-68

Contact zones of different cytotypes provide an opportunity to address evolutionary mechanisms underlying the origin, establishment and maintenance of karyological diversity at intraspecific level. We explored the fine-scale distribution of ploidy levels of Oxalis obtusa in seven mixed-ploidy sites in the Western and Northern Cape Provinces, South Africa, and searched for potential isolating barriers...

article

In vitro micronucleus assay for the analysis of total particulate matter in cigarette smoke: Comparison of flow cytometry and laser scanning cytometry with microscopy

Jianhua Yao, Qian Gao, Qili Mi, Xuemei Li, more

Mutation Research - Genetic Toxicology and Environmental Mutagenesis > 2013 > 755 > 2 > 120-125

The possible genotoxicity of the total particulate matter (TPM) in cigarette smoke has typically been evaluated using the in vitro micronucleus assay. In recent years, automated scoring techniques have been developed to replace the manual counting process in this assay. However, these automated scoring techniques have not been applied in routine genotoxicity assays for the analysis of TPM to improve...

article

Immunological responses of the mangrove oysters Crassostrea gasar naturally infected by Perkinsus sp. in the Mamanguape Estuary, Paraíba state (Northeastern, Brazil)

Fernando Ramos Queiroga, Luis Fernando Marques-Santos, Hélène Hégaret, Philippe Soudant, more

Fish and Shellfish Immunology > 2013 > 35 > 2 > 319-327

Perkinsus genus includes protozoan parasites of marine mollusks, especially bivalves. In the last four years, this parasite has been detected in mangrove oysters Crassostrea rhizophorae and Crassostrea gasar from the Northeastern region of Brazil. Hemocytes are the key cells of the oyster immune system, being responsible for a variety of cellular and humoral reactions, such as phagocytosis, encapsulation...

article

Developments in the immunophenotypic analysis of haematological malignancies

Kathy Heel, Tania Tabone, Kimberley J. Röhrig, Phillip G. Maslen, more

Blood Reviews > 2013 > 27 > 4 > 193-207

Immunophenotyping is the method by which antibodies are used to detect cellular antigens in clinical samples. Although the major role is in the diagnosis and classification of haematological malignancies, applications have expanded over the past decade. Immunophenotyping is now used extensively for disease staging and monitoring, to detect surrogate markers of genetic aberrations, to identify potential...

article

Quantification and identification of particle-associated bacteria in unchlorinated drinking water from three treatment plants by cultivation-independent methods

G. Liu, F.Q. Ling, A. Magic-Knezev, W.T. Liu, more

Water Research > 2013 > 47 > 10 > 3523-3533

Water quality regulations commonly place quantitative limits on the number of organisms (e.g., heterotrophic plate count and coliforms) without considering the presence of multiple cells per particle, which is only counted as one regardless how many cells attached. Therefore, it is important to quantify particle-associated bacteria (PAB), especially cells per particle. In addition, PAB may house (opportunistic)...

article

Lin⁻CD117⁺CD34⁺FcεRI⁺ progenitor cells are increased in chronic spontaneous urticaria and predict clinical responsiveness to anti‐IgE therapy

Katie Ridge, Barry Moran, P. Abigail Alvarado‐Vazquez, Jenny Hallgren, more

Allergy > 79 > 9 > 2423 - 2434

Background Chronic spontaneous urticaria (CSU) is a common, debilitating skin disorder characterized by recurring episodes of raised, itchy and sometimes painful wheals lasting longer than 6 weeks. CSU is mediated by mast cells which are absent from peripheral blood. However, lineage⁻CD34^hiCD117^int/hiFcεRI⁺ cells in blood have previously been shown to represent a mast cell precursor. Methods We...

article

High‐Throughput, Label‐Free Detection of DNA Origami in Single‐Cell Suspensions Using origamiFISH‐Flow

Shana Alexander, Wendy Xueyi Wang, Chung‐Yi Tseng, Travis R. Douglas, more

Small > 20 > 35 > n/a - n/a

Structural DNA nanotechnology enables custom fabrication of nanoscale devices and promises diverse biological applications. However, the effects of design on DNA nanostructure (DN)‐cell interactions in vitro and in vivo are not yet well‐characterized. origamiFISH is a recently developed technique for imaging DNs in cells and tissues. Compared to the use of fluorescent tags, origamiFISH offers label‐free...

article

Functionalizing Yeast Lipid Droplets as Versatile Biomaterials

Ankita Suri, Kevin K.Y. Hu, Tayyaba Younas, Geoff Dumsday, more

Small > 20 > 33 > n/a - n/a

Lipid droplets (LD) are dynamic cellular organelles of ≈1 µm diameter in yeast where a neutral lipid core is surrounded by a phospholipid monolayer and attendant proteins. Beyond the storage of lipids, opportunities for LD engineering remain underdeveloped but they show excellent potential as new biomaterials. In this research, LD from yeast Saccharomyces cerevisiae is engineered to display mCherry...

article

Autofluorescence lifetime flow cytometry with time‐correlated single photon counting

Kayvan Samimi, Ojaswi Pasachhe, Emmanuel Contreras Guzman, Jeremiah Riendeau, more

Cytometry Part A > 105 > 8 > 607 - 620

Autofluorescence lifetime imaging microscopy (FLIM) is sensitive to metabolic changes in single cells based on changes in the protein‐binding activities of the metabolic co‐enzymes NAD(P)H. However, FLIM typically relies on time‐correlated single‐photon counting (TCSPC) detection electronics on laser‐scanning microscopes, which are expensive, low‐throughput, and require substantial post‐processing...

article

STAT5 phosphorylation plus minimal residual disease defines a novel risk classification in adult B‐cell acute lymphoblastic leukaemia

Xiuli Xu, Zicong Huang, Chenhao Ding, Shiyu Deng, more

British Journal of Haematology > 205 > 2 > 517 - 528

The dysregulation of the Janus family tyrosine kinase‐signal transducer and activator of transcription (JAK‐STAT) is closely related to acute lymphoblastic leukaemia (ALL), whereas the clinical value of phosphorylated STAT5 (pSTAT5) remains elusive. Herein we performed a prospective study on clinical significance of flow cytometry‐based pSTAT5 in adult B‐ALL patients. A total of 184 patients were...

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Search results

Cryopreservation of chicken blastodermal cells and their quality assessment by flow cytometry and transmission electron microscopy

Polyclonal, newly derived T cells with low expression of inhibitory molecule PD-1 in tonsils define the phenotype of lymphocytes in children with Periodic Fever, Aphtous Stomatitis, Pharyngitis and Adenitis (PFAPA) syndrome

The characterisation and summer distribution of ultraphytoplankton in the Gulf of Gabès (Eastern Mediterranean Sea, Tunisia) by using flow cytometry

Joint analysis of flow cytometry data and fluorescence spectra as a non-negative array factorization problem

An efficient and rapid method for the enumeration of heterotrophic prokaryotes in coastal sediments by flow cytometry

Assessment of Daucus carota L. (Apiaceae) subspecies by chemotaxonomic and DNA content analyses

Flow cytometric assessment of microbial abundance in the near-field area of seawater reverse osmosis concentrate discharge

Impact of cold plasma on Citrobacter freundii in apple juice: Inactivation kinetics and mechanisms

Optimization of murine small intestine leukocyte isolation for global immune phenotype analysis

Recovery of soil unicellular eukaryotes: An efficiency and activity analysis on the single cell level

The spatio-ecological segregation of different cytotypes of Oxalis obtusa (Oxalidaceae) in contact zones

In vitro micronucleus assay for the analysis of total particulate matter in cigarette smoke: Comparison of flow cytometry and laser scanning cytometry with microscopy

Immunological responses of the mangrove oysters Crassostrea gasar naturally infected by Perkinsus sp. in the Mamanguape Estuary, Paraíba state (Northeastern, Brazil)

Developments in the immunophenotypic analysis of haematological malignancies

Quantification and identification of particle-associated bacteria in unchlorinated drinking water from three treatment plants by cultivation-independent methods

Lin⁻CD117⁺CD34⁺FcεRI⁺ progenitor cells are increased in chronic spontaneous urticaria and predict clinical responsiveness to anti‐IgE therapy

High‐Throughput, Label‐Free Detection of DNA Origami in Single‐Cell Suspensions Using origamiFISH‐Flow

Functionalizing Yeast Lipid Droplets as Versatile Biomaterials

Autofluorescence lifetime flow cytometry with time‐correlated single photon counting

STAT5 phosphorylation plus minimal residual disease defines a novel risk classification in adult B‐cell acute lymphoblastic leukaemia

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