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Items from 1 to 20 out of 151 results

article

Analysis of the Fibroin Solution State in Calcium Chloride/Water/Ethanol for Improved Understanding of the Regeneration Process

H.-T. Ngo, T. Bechtold

Fibres & Textiles in Eastern Europe > 2018 > Nr 6 (132) > 43--50

Shaping of fibroin protein from Bombyx mori from calcium chloride/water/ethanol solution is of high interest for the manufacturing of biocompatible structures. Potentiometric titration experiments of the dissolved state permit new insight into the solution state of fibroin as a basis for improved regeneration. Titration experiments and infrared spectroscopy of the solution state support the model...

article

A GFET Nitrile Sensor Using a Graphene‐Binding Fusion Protein

Abubaker A. Mohamed, Hironaga Noguchi, Mirano Tsukiiwa, Chen Chen, more

Advanced Functional Materials > 32 > 46 > n/a - n/a

A new route to single‐step and non‐covalent immobilization of proteins on graphene is exemplified with the first biosensor for nitriles based on a graphene field‐effect transistor (GFET). The biological recognition element is a fusion protein consisting of nitrile reductase QueF from Escherichia coli with an N‐terminal self‐assembling and graphene‐binding dodecapeptide. Atomic force microscopy and...

article

Biomolecular Regulation of Zinc Deposition to Achieve Ultra‐Long Life and High‐Rate Zn Metal Anodes

Jinlin Zhu, Wenjun Deng, Na Yang, Xianqi Xu, more

Small > 18 > 29 > n/a - n/a

Aqueous zinc‐ion batteries (ZIBs) have been extensively studied due to their inherent safety and high energy density for large‐scale energy storage. However, the practical application is significantly limited by the growing Zn dendrites on metallic Zn anode during cycling. Herein, an environmental biomolecular electrolyte additive, fibroin (FI), is proposed to guide the homogeneous Zn deposition and...

article

Biomedical applications of silkworm (Bombyx Mori) proteins in regenerative medicine (a narrative review)

Mohammad Hossein Khosropanah, Mahdi Alizadeh Vaghasloo, Mehdi Shakibaei, Anna‐Lena Mueller, more

Journal of Tissue Engineering and Regenerative Medicine > 16 > 2 > 91 - 109

Silk worm (Bombyx Mori) protein, have been considered as potential materials for a variety of advanced engineering and biomedical applications for decades. Recently, silkworm silk has gained significant importance in research attention mainly because of its remarkable and exceptional mechanical properties. Silk has already been shown to have unique interactions with cells in tissues through bio‐recognition...

article

Transcriptomic analysis of the bagworm moth silk gland reveals a number of silk genes conserved within Lepidoptera

Takuya Tsubota, Taiyo Yoshioka, Akiya Jouraku, Takao K. Suzuki, more

Insect Science > 28 > 4 > 885 - 900

Lepidopteran insects produce cocoons with unique properties. The cocoons are made of silk produced in the larval tissue silk gland and our understanding of the silk genes is still very limited. Here, we investigated silk genes in the bagworm moth Eumeta variegata, a species that has recently been found to produce extraordinarily strong and tough silk. Using short‐read transcriptomic analysis, we identified...

article

Potential use of silkworm gut fiber braids as scaffolds for tendon and ligament tissue engineering

Ana Pagán, Salvador D. Aznar‐Cervantes, José Pérez‐Rigueiro, Luis Meseguer‐Olmo, more

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 107 > 7 > 2209 - 2215

Tendon and ligament tissue engineering require scaffolds for the treatment of various conditions in the medical field. These must meet requirements such as high tensile strength, biocompatibility, fast and stable repair and a rate of degradation that allows the repair of the damaged tissue. In this work, we propose the use of silkworm gut fiber braids as materials to temporarily replace and repair...

article

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Weiwei Wang, Yanni Yu, Yi Jiang, Jing Qu, more

Journal of Tissue Engineering and Regenerative Medicine > 13 > 5 > 715 - 728

Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin‐1 (Ang‐1) dual gene coexpression vector that encoded green fluorescent protein (GFP) was constructed from an arginine–glycine–aspartic acid‐modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze‐drying...

article

Scaffolding kidney organoids on silk

Ashwani Kumar Gupta, Jeannine M. Coburn, Jessica Davis‐Knowlton, Erica Kimmerling, more

Journal of Tissue Engineering and Regenerative Medicine > 13 > 5 > 812 - 822

End stage kidney disease affects hundreds of thousands of patients in the United States. The therapy of choice is kidney replacement, but availability of organs is limited, and alternative sources of tissue are needed. Generation of new kidney tissue in the laboratory has been made possible through pluripotent cell reprogramming and directed differentiation. In current procedures, aggregates of cells...

article

The Energy Requirements for Flow‐Induced Solidification of Silk

James Sparkes, Chris Holland

Macromolecular Bioscience > 19 > 3 > n/a - n/a

Natural silk spinning has undergone strong selection for resource efficiency and thus presents a biomimetic ideal for fiber production. Industrial replication of natural silk fibers would enable access to low energy, cost‐efficient processing, but is hampered by a lack of understanding surrounding the conversion of liquid feedstock into a solid fiber as a result of flow. Previously, shear stress,...

article

Chinese Oak Tasar Silkworm Antheraea pernyi Silk Proteins: Current Strategies and Future Perspectives for Biomedical Applications

Simone S. Silva, Banani Kundu, Shenzhou Lu, Rui L. Reis, more

Macromolecular Bioscience > 19 > 3 > n/a - n/a

Chinese nonmulberry temperate oak tasar/tussah, Antheraea pernyi (Ap) silk is a natural biopolymer that has attracted considerable attention as a biomaterial. The proteinaceous components of Ap silk proteins, namely fibroin and sericin may represent an alternative over mulberry Bombyx mori silk proteins. In fact, the silk fibroin (SF) of Ap is rich in Arginyl‐Glycyl‐Aspartic acid (RGD) peptides, which...

article

Silk fibroin hydrogels for potential applications in photodynamic therapy

Jose Eduardo U. Rojas, Barbara B. Gerbelli, Anderson O. Ribeiro, Iseli L. Nantes‐Cardoso, more

Biopolymers > 110 > 2 > n/a - n/a

In this study, we prepared translucid hydrogels with different concentrations of silk fibroin, extracted from raw silk fibers, and used them as a matrix to incorporate the photosensitizer 5‐(4‐aminophenyl)‐10,15,20‐tris‐(4‐sulphonatophenyl) porphyrin trisodium for application in photodynamic therapy (PDT). The hydrogels obtained were characterized by rheology, spectrophotometry, and scattering techniques...

article

Development of regenerative and flexible fibroin‐based wound dressings

Angelica Panico, Federica Paladini, Mauro Pollini

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 107 > 1 > 7 - 18

Skin injuries represent a health problem with consequences in terms of morbidity, disability and life quality. Numerous strategies have been developed for the treatment of wounds, including skin substitutes, biomembranes, scaffolds, and smart dressings. The excellent properties of fibroin can be exploited for the development of advanced wound dressing biomaterials, aiming at promoting the wound healing...

article

Effect of Silk Fibroin on Neuroregeneration After Traumatic Brain Injury

M. M. Moisenovich, E. Y. Plotnikov, A. M. Moysenovich, D. N. Silachev, more

Neurochemical Research > 2019 > 44 > 10 > 2261-2272

Traumatic brain injury is one of the leading causes of disability among the working-age population worldwide. Despite attempts to develop neuroprotective therapeutic approaches, including pharmacological or cellular technologies, significant advances in brain regeneration have not yet been achieved. Development of silk fibroin-based biomaterials represents a new frontier in neuroregenerative therapies...

article

Altering Silk Film Surface Properties through Lotus‐Like Mechanisms

Eileen S. Lintz, Christoph Neinhuis, Thomas Scheibel

Macromolecular Materials and Engineering > 303 > 4 > n/a - n/a

The nonwetting and self‐cleaning properties of the lotus depend on microscale and nanoscale roughness provided in part by a covering of epicuticular waxes that crystalize on the surface of its leaves. Wax deposition is driven by the evaporation of water, which carries waxes to the surface as it moves through the epidermis and cuticle. If the wax layer is damaged, repair occurs through the same mechanism...

article

Apoptosis of posterior silk gland of Bombyx mori during spinning period and the role of PI3K/Akt pathway

Jia‐Huan Hu, Xiao‐Yu Cheng, Jin‐Xin Li, Bin Xue, more

Archives of Insect Biochemistry and Physiology > 98 > 1 > n/a - n/a

Bombyx mori is an economic insect of the Lepidoptera. Its posterior silk gland (PSG) is an important organ for fibroin synthesis. In order to study the occurrence of apoptosis in PSG and the role of PI3K/Akt signaling pathway during spinning period, changes in morphology of silk gland, expressions of fibroin components Fib‐H, Fib‐L and P25 and Akt, TOR2, P70S6K and S6 in PI3K/Akt pathway, expressions...

article

Bioresorbable Scaffolds Based on Fibroin for Bone Tissue Regeneration

M. S. Kotliarova, A. Yu. Arkhipova, A. M. Moysenovich, D. A. Kulikov, more

Moscow University Biological Sciences Bulletin > 2017 > 72 > 4 > 190-195

Using the tissue-engineered constructs based on scaffolds that imitate the extracellular matrix of living tissues unveils new opportunities in the treatment of various pathologies and injuries associated with tissue and organ damage. Silk fibroin of silkworm Bombyx mori is a biocompatible and bioresorbable polymer with high mechanical strength and elasticity that allows creating scaffolds on its basis...

article

Fibroin/dodecanol floating solidification microextraction for the preconcentration of trace levels of flavonoids in complex matrix samples11Abbreviations: F/D-FSME, fibroin/dodecanol floating solidification microextraction; HPLC, high performance liquid chromatography; UV, ultraviolet detection; EF, enrichment factor; TCM, traditional Chinese medicine; LPE, liquid phase extraction; SPE, solid phase extraction; LPME, liquid phase microextraction; SPME, solid phase microextraction; SFODLPME, solidification of floating organic droplet liquid phase microextraction; D-FSME, dodecanol floating solidification microextraction.

Xuan Chen, Jie Li, Shuang Hu, Xiaohong Bai, more

Journal of Chromatography B > 2018 > 1072 > C > 17-24

A new fibroin/dodecanol floating solidification microextraction, coupled with high performance liquid chromatography, was developed and applied for enrichment and quantification of the trace flavonoids in traditional Chinese medicine and biological samples. Also, fibroin sensibilization mechanism was described, and influence of sample matrix to enrichment factor was investigated. In this method, a...

article

Bilayer Amniotic Membrane/Nano-fibrous Fibroin Scaffold Promotes Differentiation Capability of Menstrual Blood Stem Cells into Keratinocyte-Like Cells

Maryam Fard, Maryam Akhavan-Tavakoli, Sayeh Khanjani, Sona Zare, more

Molecular Biotechnology > 2018 > 60 > 2 > 100-110

The skin provides a dynamic barrier separating and protecting human body from the exterior world, and then immediate repair and rebuilding of the epidermal barrier is crucial after wound and injury. Wound healing without scars and complete regeneration of skin tissue still remain as a clinical challenge. The demand to engineer scaffolds that actively promote regeneration of damaged areas of the skin...

article

Photocurable Hydrogels Containing Spidroin or Fibroin

I. V. Bessonov, M. S. Kotliarova, M. N. Kopitsyna, A. V. Fedulov, more

Moscow University Biological Sciences Bulletin > 2018 > 73 > 1 > 24-27

Photocurable biocompatible hydrogels were fabricated from methacrylated gelatin and silk proteins (a recombinant analogue of spidroin from Nephila clavipes spider web and fibroin from the cocoons of the silkworm Bombyx mori). These polymers are widely applicable in tissue engineering due to their biocompatibility and biodegradability. Hydrogels were fabricated using two different methods that allowed...

article

Straining flow spinning: Simplified model of a bioinspired process to mass produce regenerated silk fibers controllably

Rodrigo Madurga, Gustavo V. Guinea, Manuel Elices, José Pérez-Rigueiro, more

European Polymer Journal > 2017 > 97 > C > 26-39

This paper describes a method of molecular self-assembly by using two interacting streams for the production of bioinspired and biocompatible fibers. A first stream of an aqueous solution (dope) of polymer molecules (fibroins) is extruded from a capillary. The end of the capillary is surrounded by a focusing fluid which is miscible with the dope solution. The interaction between the dope solution...

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Search results

Analysis of the Fibroin Solution State in Calcium Chloride/Water/Ethanol for Improved Understanding of the Regeneration Process

A GFET Nitrile Sensor Using a Graphene‐Binding Fusion Protein

Biomolecular Regulation of Zinc Deposition to Achieve Ultra‐Long Life and High‐Rate Zn Metal Anodes

Biomedical applications of silkworm (Bombyx Mori) proteins in regenerative medicine (a narrative review)

Transcriptomic analysis of the bagworm moth silk gland reveals a number of silk genes conserved within Lepidoptera

Potential use of silkworm gut fiber braids as scaffolds for tendon and ligament tissue engineering

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Scaffolding kidney organoids on silk

The Energy Requirements for Flow‐Induced Solidification of Silk

Chinese Oak Tasar Silkworm Antheraea pernyi Silk Proteins: Current Strategies and Future Perspectives for Biomedical Applications

Silk fibroin hydrogels for potential applications in photodynamic therapy

Development of regenerative and flexible fibroin‐based wound dressings

Effect of Silk Fibroin on Neuroregeneration After Traumatic Brain Injury

Altering Silk Film Surface Properties through Lotus‐Like Mechanisms

Apoptosis of posterior silk gland of Bombyx mori during spinning period and the role of PI3K/Akt pathway

Bioresorbable Scaffolds Based on Fibroin for Bone Tissue Regeneration

Bilayer Amniotic Membrane/Nano-fibrous Fibroin Scaffold Promotes Differentiation Capability of Menstrual Blood Stem Cells into Keratinocyte-Like Cells

Photocurable Hydrogels Containing Spidroin or Fibroin

Straining flow spinning: Simplified model of a bioinspired process to mass produce regenerated silk fibers controllably

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