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The prevalence of bovine viral diarrhea (BVD) virus infection on the basis of serological testing of cattle has been evaluated. Blood samples were analyzed in micro-seroneutralization test for the presence of BVD virus neutralizing antibodies against 1000 TCID₅₀ of NADL reference strain. Neutralizing antibodies were found in 83 per cent of animals tested. The percentage of seropositive animals was...
RT-PCR was evaluated as a diagnostic tool for BVDY detection. First, five sets of primers were checked for their diagnostic ability to amplify the number of field isolates. Only primers within the 5'UTR region amplified all field isolates as well as all reference laboratory strains. Next, sensitivity of the test was evaluated. This was equal to 1 TCID₅₀ of the virus. lt was concluded that RT-PCR can...
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