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Electron microscopy has been applied widely to study the interaction of nanomaterials with proteins, cells and tissues at nanometre scale. Biological material is most commonly embedded in thermoset resins to make it compatible with the high vacuum in the electron microscope. Room temperature sample preparation protocols developed over decades provide contrast by staining cell organelles, and aim to...
What one may see upon microscopic examination of sections of specimens of human and animal tissues is not always related to the normal histology or pathology of the tissue in question. Defects or abnormalities in tissue sections may result from the faulty processing of the tissue specimens. These are referred to as artefacts. Some artefacts are easily distinguished from normal or pathological tissue...
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