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Environmental pollution has become one of the most prominent problems in the world. In the present study, a PAH pollutant degrading bacterium NAPL was isolated from the activated sludge of a wastewater treatment plant in Zibo city, China, with the selective naphthalene-mineral salts medium. Morphological studies revealed NAPL was a short-rod gram negative bacterium. Physiological and biochemical experiments...
Chitin deacetylase (CDA) is an enzyme that catalyzes the hydrolysis of acetamine groups of N-acetyl-D-glucosamine in chitin. Chitin deacetylase converting chitin to chitosan in fungal cell walls. A total of 208 strains was isolated from soil samples in the riverbank of Xiangjiang River and an island of Zhanjiang. 12 CDA producing strains was founded by color reaction that including three fungus, two...
The DPV UL46 gene( GenBank accession No. EU195108) isolated by our laboratory was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmid pMD18-T-UL46 was identified by PCR, restriction enzymes (BamHI+XhoI), sequencing and nucleic acid dot-hybridization. Then the gene was analyzed by bioinformatics tools. The results showed that a 2220bp complete open reading frame of 739 amino acids...
Nitrite reductase (nirS) is a key enzyme of denitrification catalyzing the one electron reduction of nitrite (NO2-) to nitrogen monoxide (NO).In this study, nirS gene was cloned from Pseudomonas aeruginosa HGP9 strain. The phylogenetic tree was constructed and the secondary structure was predicted by bioinformatics. Results showed that nirS gene was 99.8% similar to the nitrite reductase from Pseudomonas aeruginosa...
The UL27 open reading frame of the Duck Plague virus encodes glycoprotein B, the molecular characteristic of this gene was analyzed with bioinformatics software. The results indicated that this gene encodes an estimated 1000 putative proteins, contains the conserved domain of the Herpesvirus UL27-like protein. Similarity comparisons of DPV CHv strain gB protein sequences with those of 20 known herpesviruses...
Duck enteritis virus (DEV) is an alphaherpesvirinae that causes an acute, contagious, and fatal disease. In this study, the DEV-ULS3 gene from Key Laboratory of Animal Disease and Human Health of Sichuan Province was cloned and sequenced. Nucleotide sequence analysis revealed a 1032 bp open reading frame (ORF) theoretically encoding a 343-amino acid polypeptide was homologous to UL53 gene of alphaherpesvirinaes...
The focus of this paper is on the newly identified UL55 gene of DEV in our laboratory.Available bioinformatic tools were used to describe its molecular characteristics. A identified nucleotide sequence about 561bp of DEV UL55 ,consisting a complete ORF, was deduced to encode a protein with 186 amino acids.The 20.7981 kDa protein whose singnal peptide and transmembrane region are both absent. Codon...
Lipase activity is popular in staphylococci. We described here that a 781-bp consensus lipase gene sequence of Staphylococcus caprae TCCC 11546, which encoded a deduced polypeptide of 260 amino acid residues, was cloned using the degenerate primers. The amino acid sequence alignment and the phylogenetic tree revealed that this 260-amino acid partial lipase of S. caprae was highly homologous from 55...
The article introduces the authors'research of an optimum method on the cultivation conditions of a flocculant- producing Strain. Using single factor experiments and orthogonal test, the influence of temperature, PH value and rotational speed on synthesis of microbial flocculants, and optimized culture conditions were studied; 16S gene of the strain was amplified and sequenced.The sequencing was BLAST...
In this paper we present novel web services offered by the StrainInfo.net bioportal. This portal integrates information in the domain of microbiology and offers a uniform web interface to a multitude of data providers. By providing web services, the integration results of StrainInfo.net become available for automated processing. Several classes of web services are implemented and some interesting...
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