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After 27 years of storage in liquid nitrogen, we managed to restore the growth of suspension cell culture of alfalfa (Medicago sativa L.). The influence of factors affecting viability of alfalfa cells in vitro was investigated together with cytogenetic and physiological characteristics after their storage at the temperature of liquid nitrogen (−196°C). As a result of freezing/thawing, approximately...
This paper analyses the use of Electrical Impedance Spectroscopy (EIS) to efficiently monitor cryoprotectant concentrations in cryopreservation protocols. The proposed technique can improve methods such as Liquidus Tracking (LT), allowing vitrification without exposing tissues to damaging concentrations of cryoprotectant at relatively high temperatures, and avoiding rapid temperature changes. This...
The intracellular hepatitis B surface antigen (HBsAg) content per cell was significantly increased by 7.2-fold in the culture of recombinant CHO cells with 1.5% dimethyl sulfoxide (DMSO), while the HBsAg production and specific productivity were only improved by 70% and 3.2-fold, respectively. The significant accumulation of HBsAg within rCHO cells by DMSO stimulation was testified with flow cytometry...
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