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A rapid, sensitive and accurate TLC method has been developed for estimation of various pharmaceutical agents, such as piroxicam, tenoxicam, celecoxib and rofecoxib in human whole blood and urine. TLC analysis was performed on silica gel F 254 plates with ethyl acetate-toluene-butylamine (2 : 2 : 1, v/v/v) and chloroform-acetone-toluene (6 : 2.5 : 1, v/v/v) as mobile phases. Densitometric...
Different thin-layer chromatography (TLC) systems were evaluated for analysis of 21 biologically important essential and nonessential amino acids in complex mixtures such as biological tissues and fluids. Amino acids were visualized on the layers by derivatization with ninhydrin reagent, and RFvalues were determined by slit-scanning densitometry. The five systems found to be most useful for analysis...
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