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Background Ovine footrot is a highly contagious bacterial disease of sheep, costing the Australian sheep industry millions of dollars annually. Dichelobacter nodosus, the causative agent of footrot, is a gram-negative anaerobe classed into virulent and benign strains as determined by thermostability of their respective protesases. Current methods for detection of D. nodosus are difficult and time-consuming,...
The aim of the present study was to investigate the effect of exogenous leptin on somatotrophic axis regulation in prepubertal female lambs under conditions of acute undernutrition (72 h fasting). The analyses in fasted sheep revealed enhancement of immunoreactive (ir) somatostatin stores in perikarya of the periventricular (PEV) nucleus and in terminals of the median eminence (ME) (P<0.001), enhancement...
Application of real-time PCR using Taqman probe was tested for jaagsiekte sheep retrovirus (JSRV) detection. Sensitivity of real-time PCR and hemi-nested PCR methods was compared using plasmid DNA. The methods, along with RT PCR and real-time RT PCR, were tested for the possibility of JSRV genome (LTR region) detection in biological material from experimentally and naturally infected sheep. The experimental...
Real-time RT-PCR (rRT-PCR) for the detection of bluetongue virus (BTV) in EDTA treated blood samples taken from BTV infected animals was described. A combination of two primer sets (representing eastern and western BTV serotypes) and two Taqman probes specific for a highly conserved region in BTV RNA segment 1 were used. The assay detected the viral RNA in blood samples collected from seropositive...
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