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The trehalase (TRE) is one of enzymes related to trehalose catabolism in plant. On the basis of previous experiments, we are trying to inhibit the expression of trehalase gene through RNA interference technology in order to achieve the purpose of increasing the accumulation of trehalose in plant. So the fragments of TRE gene and RNAi-TRE have been obtained from Arabidopsis seedling by using RT-PCR...
Polymerase Chain Reaction (PCR) is a technique which relies on a polymerase enzyme to amplify a short DNA sequence (target analyte) by replicating it through a series of thermal cycles. Determining the initial number of target analytes is often the goal of quantitative PCR (QPCR). QPCR is used in several applications including detection and quantification of nucleic acids, viral load detection, and...
The molecular probes and associated instrumentation necessary to perform genetic analyses are typically expensive, complex, and prone to error. While techniques such as real-time polymerase chain reaction (PCR) and gene expression analysis have provided a wealth of information previously unattainable, their utility in clinical diagnostics has yet to be realized due to the aforementioned limitations...
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