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The enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) from P. aeruginosa is required for the biosynthesis of two bacterial exopolysaccharides: alginate and lipopolysaccharide (LPS). Both of these molecules play a role in the virulence of P. aeruginosa, an important human pathogen known for its ability to develop antibiotic resistance and cause chronic lung infections in cystic fibrosis patients...
A milder method for encapsulating viable bacterial cells in κ-carrageenan was developed. A 1% (w/v) κ-carrageenan concentration amended with clay and skim milk was used as a lower-cost alternative to alginate for potential introductions of bacteria into soil. Survival of lac-lux marked Pseudomonas aeruginosa UG2Lr cells during long-term storage in dried κ-carrageenan and alginate beads at 4 ...
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