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A well-structured and controlled design methodology, along with a supporting hierarchical design system, has been developed to optimally support the development effort on several programs requiring gate array and semi custom Very Large Scale Integration (VLSI) design. In this paper, we will present an application of VLSI in System Biology. This work examines signaling networks that control the survival...
Objective To investigate the relationship between caspase-3 expression and apoptosis in the lung adenocarcinoma cell line A-549 that was transfected with the adenovirus-mediated FasL gene. Methods The human lung adenocarcinoma cell line A-549 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured using flow cytometry and...
Cellular decision making in differentiation, proliferation or cell death is mediated by molecular signalling processes, which control the regulation and expression of genes. Vice versa, the expression of genes can trigger the activity of signalling pathways. We introduce and describe a statistical method called dynamic nested effects model (D-NEM) for analyzing the temporal interplay of cell signalling...
This paper presents an image processing-based approach for the assessment of living cell apoptosis. The approach uses deformable models in tracing the changes in cell shapes in accordance to possible apoptotic cell death. Stochastic controlled energy function is used to drive the evolution of the deformable model. As an application for the proposed approach, it is applied for detecting the morphological...
Many studies have focused on the biological responses of macrophages, osteoblasts, and osteoclasts cells after knee joint replacements. However, synoviocytes are also in close contact with wear particles within the joint cavity; their responses would influence other cells, and they may serve as inflammatory cells around the knee joint. Therefore, in this study we have examined the affects of 1~2 Vm...
Aluminum (Al) toxicity is the major factor in acidic soil that limits crop production. Our results showed that Al could disturb organelle morphology and distribution in an oxidative stress-dependent manner.
While antitumor immunological responses have been mainly associated with necrosis, immune responses associated with apoptosis have been suggested recently. In this study, the relationship between cell death and its interaction with macrophage were studied using Photofrin-PDT induced necrosis and apoptosis.
This paper represents a study on the effect of electrical pulses on adult stem cells, especially on proliferation control and also as a method of delivering molecules inside the cytoplasm. Mesenchymal stem cells have been targeted for this study primarily because of their ability to home towards tumor cells, making the possibility to generate targeted tumor therapy more realistic. Human adult bone...
Cadmium (Cd) and Copper (Cu) are common transition metals found in the environment. The toxic effects of Cd on human health have received considerable concerns. Cu is the trace essential element, however, excess amounts of copper causes toxic effects to life. To have a better understand of the impacts of heavy metals exposure on human beings as well as other animals, many bioassay systems have been...
The background of this study is Tanshinone was thought to be a potential antitumor drug. In this study, we investigated the apoptosis of hepatoma HepG2 induced by Tanshinone II-A as well as the involved molecules. The inhibitory effects on cell proliferation of Tanshinone II-A were assessed by MTT method. DNA fragmentation was evaluated by agarose gel electrophoresis. Cell cycle and apoptotic rates...
To investigate the changes of immuneparameter induced by the infection of Streptococcus suis serotype 2 (SS2) in mini-swine model. The pigs were inoculated with 1 times 107 CFU ml-1/kg of two SS2 strains (ZY05719 and HA9801). Typical clinical symptoms and pathological features were confirmed after infection. Bacterial content in certain organs and changes of white blood cell counts were measured;...
Deterioration of peach fruit quality because of split-pit formation had bad influence on market. Though many studies have mentioned the mechanism of split-pit formation, further studies are still needed to uncover the development mechanism of endocarp cell during pit hardening process, especially the Programmed cell death (PCD) process of peach endocarp cell. Here, the development of the peach endocarp...
AIM: In the present study, murine A549 Lung cancer cells were provided Lung cancer with different thermal dose invitro and invivo, thereafter we investigated the apoptosis, necrosis rates, and intratumoral microvessel density (MVD) to determine that microvessel damage plays an important role in the tumoricidal effect of Lung cancer. Methods: A549 Lung cancer cells were inoculated in the right hind...
The objective of this study is to investigate the relationship between caspase-3 expression and apoptosis in the gastric adenocarcinoma cell line SGC-7901 transfected with the adenovirus-mediated FasL gene. The human gastric adenocarcinoma cell line SGC-7901 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured by flow...
Cardiomyocyte death is a critical risk factor in a variety of cardiovascular diseases. At the level of individual cardiomyocyte, cell adhesion alters at the early phase of ongoing death. In the previous study, we developed the impedance-sensing technique for real-time monitoring cardiomyocyte adhesion. In this work, we demonstrated the application of our impedance-sensing technique in regulating cell...
A mathematical model of tumor angiogenesis, regulated by vascular endothelial growth factor (VEGF) and angiopoietins (Ang-1, Ang-2), is presented. The Overture numerical simulation of this model is developed, and the result analysis shows the characteristics of the density changing of endothelial cells, vascular endothelial growth factor and angiopoietins in the extra-cellular matrix in tumor angiogenesis...
Cell growth and cell division are fundamental aspects of cell behavior in all organisms. Recent insights from many model organisms have shed light on the molecular mechanisms that control cell growth and cell division. A significant body of evidence has now been accumulated, showing a direct link between deregulation of components of cell cycle machinery and cancer. Cancer can be described as a genetic...
Antibiotic resistance is a rapidly evolving problem that is not being adequately met by new antimicrobial drugs. Thus, there is a pressing need for effective antibacterial therapies that can be adapted against antibiotic-resistant bacteria. Here, we engineered synthetic bacteriophage to combat antibiotic-resistant bacteria by overexpressing proteins and attacking gene networks which are not directly...
While stem cells hold great promise as a means of treating otherwise incurable disease due to their characteristic ability to both self-renew and differentiate, they can also play a role in disease. Chronic myeloid leukemia (CML) arises in CD34+ hematopoietic stem, and is known resistant to the tyrosine kinase inhibitors imatinib and dasatinib. Understanding the mechanism which rescue particular fractions...
Traumatic brain injury (TBI) is caused by in response to mechanical loading of the head. An extended biological cascade is initiated by the deformation and results in delayed cell death and dysfunction. Using an in vitro model of TBI based on organotypic brain slice cultures, we have quantitatively studied the relationship between tissue deformation and outcome. In the current study, we asked the...
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