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We examined the influence of incubation time or cytosolic protein concentration on the metabolite production of 5-fluorouracil (5FU). Although the activity of dihydropyrimidine dehydrogenase (DPDase) from rat liver is considered to be retained for up to 60 min, the production rate of 5FU metabolites was reduced, probably due to depletion of the substrate in 40 μmol/l and lower concentration of 5FU...
This work comprises the results of comparative research on the determining the number of heterotrophic bacteria in underground waters of one of the largest Aquifers without isolation from the surface by an impermeable layer on the Mazurian Lake District. Modified Walters and Schwartz's medium and Groundwater Agar prepared on underground water were used for research. The investigations were carried...
The 51 Cr-release assay is mostly applied to detecting the cytotoxic activity of CD8 + T cells, and little is known about the activity of CD4 + T cells. Therefore, the correlation between the cytotoxic activity of CD4 + or CD8 + T cells and the incubation period with autologous tumor cells was analyzed by two methods. The incubation periods were 4 and...
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