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Parameters for biolistic transfer of viroid nucleic acids using a Helios Gene Gun device were assayed. The main achievement of this method is high efficiency of inoculation with linear monomeric viroid cDNAs and RNAs. This greatly facilitates the study of mutated sequence variants, viroid libraries and mixed populations. The lower limits for efficient inoculation of monomeric cDNA fragments with the...
The acyclic homoterpene 4,8-dimethylnona-1,3,7-triene is a metabolite of (3S)-nerolidol. The absolute configuration of the precursor was established by GC-MS analysis of the molecular ion of the homoterpene produced after feeding a mixture consisting of equal amounts of (3S)-[12- 2 H 3 , 13- 3 H 3 ]- and (3R)-[4- 2 H 2 , 15- 2 H 3 ]nerolidol...
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