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Cell suspension cultures of Solanum malacoxylon cultivated in standard B5 medium containing 1 mM CaCl 2 accumulated larger amounts of both vitamin D 3 (cholecalciferol) and 25-OH D 3 (25-hydroxy-cholecalciferol) than cells in medium containing 10 mM CaCl 2 . The amount of vitamin D 3 in calcium-free medium was similar to that in standard medium, while the 25-OH...
The presence of cholesterol, 7-dehydrocholesterol, cholecalciferol (vitamin D 3 ) and its hydroxylated metabolites, 25-hydroxycholecalciferol (25-OH D 3 ) and 1α,25-dihydroxycholecalciferol (1α,25-diOH D 3 ), was demonstrated in cell cultures derived from sterile leaves of Solanum malacoxylon. When calli were grown in the dark, 7-dehydrocholesterol was found and identified...
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