Scanning transmission X‐ray microscopy, especially in combination with X‐ray fluorescence detection (STXM‐XRF) in the soft X‐ray energy range, is becoming an increasingly important tool for life sciences. Using X‐ray fluorescence detection, the study of biochemical mechanisms becomes accessible. As biological matrices generally have a low fluorescence yield and thus a low fluorescence signal, high detector efficiency (e.g. large solid angle) is indispensable for avoiding long measurement times and radiation damage. Here, the new AnImaX STXM‐XRF microscope equipped with a large solid angle of detection enabling fast scans and the first proof‐of‐principle measurements on biomedical samples are described. In addition, characterization measurements for future quantitative elemental imaging are presented.