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High‐quality spermatozoa are essential for intracytoplasmic sperm injection. However, there is still a lack of noninvasive, objective tools that provide biochemical characteristics to select high‐quality spermatozoa in clinical settings. In this work, a facile yet efficient approach was proposed, rendering the live sperm cell with its head being attached on the surface of a substrate, which allows...
Micro‐Raman spectroscopy can find the carbon isotopic ratio of CO2 fluid from the ratio of intensity or area of a 13CO2 peak to that of a 12CO2 peak. We examined the precisions of carbon isotopic ratios (δ13C) of CO2 at constant room temperature and pressure of 10–150 MPa. Measurement of the intensity ratio has precision of 2.8–8.7‰, which is better than that of the area ratio of 4.5–14.7‰. We also...
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