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Optical trapping‐based force spectroscopy was used to measure the frequency‐dependent DEP forces and DEP crossover frequencies of colloidal polymethyl methacrylate spheres and clusters. A single sphere or cluster, held by an optical tweezer, was positioned near the center of a pair of gold‐film electrodes where alternating current elecroosmosis flow was negligible. Use of amplitude modulation and...
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