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The application of pressurized capillary electrochromatography (pCEC) coupled with ultra violet (UV) detection has been investigated for the production of global metabolite profiles from human plasma, and its capabilities of classifying pancreatic cancer patients. The pCEC separation of plasma samples was performed on a RP column with gradient elution. The applied voltage, detection wavelength and...
The performance of Cibacron Blue dye (HiTrapBlue or Affigel Blue) in depleting albumin from plasma, as a pre‐treatment for biomarker searching in the low‐abundance proteome, is here assessed. It is shown that (i) co‐depletion of non‐albumin species is an ever‐present hazard; (ii) the only proper eluant able to release quantitatively the proteins bound to the dye is boiling 4% SDS‐25 mM DTT, an ion...
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