We show the formation of macroscopic ATP‐concentrations in an agarose gel and demonstrate that these gradients can be sustained in time at the expense of the consumption of a chemical fuel. The approach relies on the spatially controlled activation of ATP‐producing and ATP‐consuming reactions through the local injection of enzymes in the matrix. The reaction‐diffusion system is maintained in a stationary non‐equilibrium state as long as chemical fuel, phosphocreatine, is present. The reaction‐diffusion system is coupled to a supramolecular system composed of monolayer protected gold nanoparticles and a fluorescent probe. As a result of this coupling, fluorescence signals emerge spontaneously in response to the ATP‐concentration gradients. We show that the approach permits the rational formation of complex fluorescence patterns that change over time as a function of the evolution of the ATP‐concentrations present in the system.