Soil samples were collected from the rhizosphere of faba bean (Vicia faba L.) in Khuzestan province, south-western Iran. Many of the localities were positive for the presence of Aphelenchus avenae. The morphological and morphometric characters of the two Iranian populations are discussed and illustrated based on morphological and morphometrics data. Additionally, sequences of the D2-D3 expansion segments of 28S rRNA gene for two populations were used for molecular phylogenetic analysis. One population was without male and formed a separate branch in the phylogenetic tree. Results indicate high genetic variation among A. avenae populations from different geographic regions.