Populus euphratica has been used as a plant model to study resistance against salt and osmotic stresses, with recent studies having characterized the tonoplast and the plasma membrane ATPases, and two Na+/H+ antiporters, homologs of the Arabidopsis tonoplast AtNHX1, were published in databases. In the present work we show that P. euphratica suspension-cultured cells are highly tolerant to high salinity, being able to grow with up to 150 mM NaCl in the culture medium without substantial modification of the final population size when compared to the control cells in the absence of salt. At a salt concentration of 300 mM, cells were unable to grow but remained highly viable up to 17 days after subculture. The addition of a 1-M-NaCl pulse to unadapted cells did not promote a significant loss in cell viability within 48 h. In tonoplast vesicles purified from cells cultivated in the absence of salt and from salt-stressed cells, vacuolar H+-pyrophosphatase (V-H+-PPase) seemed to be the primary tonoplast proton pump; however, there appears to be a decrease in V-H+-PPase activity with exposure to NaCl, in contrast to the sodium-induced increase in the activity of vacuolar H+-ATPase (V-H+-ATPase). Despite reports that in P. euphratica there is no significant difference in the concentration of Na+ in the different cell compartments under NaCl stress, in the present study, confocal and epifluorescence microscopic observations using a Na+-sensitive probe showed that suspension-cultured cells subject to a salt pulse accumulated Na+ in the vacuole when compared with control cells. Concordantly, a tonoplast Na+/H+ exchange system is described whose activity is upregulated by salt and, indirectly, by a salt-mediated increase of V-H+-ATPase activity.