The physical and numerical models were combined to analyze flow-induced vibration response of Qingshuihe River underwater horizontal gate in this paper; the pressures acting on the gate were divided into two parts: the fluctuating pressure of flow on the stationary gate and the hydrodynamic pressure caused by the gate vibration, which is additional pressure induced by disturbed flow. The temporal-spatial correlation of fluctuating pressures obtained by model experiments between different nodes was analyzed. In the study the coherence function is defined in frequency domain with consideration of different levels of correlation for different frequency components, and the nodal load of the fluctuating pressure could be obtained. A new distribution of additional mass with considering radial vibration of the gate is adopted as equivalent hydrodynamic pressure. Based on random vibration theory, the flow-induced vibration response of the gate was obtained. The results provide the reliable reference evidence for structural dynamic design of the gate and show that the hydrodynamic stability of the gate can meet the requirement. On the other hand, it is shown that this method is reasonable and feasible.